About the Workshop: Real-time PCR (qPCR) is a powerful and versatile technique for detecting and quantifying nucleic acids. This workshop aims to provide participants with theoretical knowledge and hands-on experience in using qPCR for detecting pathogens and point mutations associated with human diseases. Designed for researchers, clinicians, and students, the workshop enhances understanding and technical expertise in qPCR applications.

Key Highlights:

• Introduction to real-time PCR technology
• Principles of probe-based and dye-based detection methods
• Optimization of qPCR assays
• Analysis of pathogen DNA/RNA detection
• Detection of human disease-associated point mutations
• Troubleshooting and data interpretation

Who Should Attend?

• Clinicians and diagnostic lab personnel
• Postgraduate and doctoral students in life sciences
• Researchers in molecular biology and genetics

Workshop Agenda:

1. Session 1: Basics of Real-Time PCR (Theory)
   • Introduction to qPCR
   • Types of chemistries: SYBR Green and TaqMan probes
2. Session 2: Applications of qPCR in Pathogen Detection (Practical)
   • Hands-on training in detecting bacterial and viral DNA/RNA
3. Session 3: Detection of Human Disease Point Mutations (Practical)
   • Allele-specific qPCR
   • Mutation analysis and result interpretation
4. Session 4: Data Analysis and Troubleshooting (Interactive Session)
   • Understanding qPCR efficiency and melt curve analysis
   • Common pitfalls and troubleshooting techniques
5. Session 5: Real-Time PCR-Based Detection of Fusion Transcripts
   • Introduction to detecting gene fusions using qPCR
   • Practical session on RNA extraction and cDNA synthesis

13th-February-2025-Workshop-4