Introduction:

We are excited to invite you to a comprehensive workshop on Next-Generation Sequencing (NGS), focusing on its applications in diagnostics and research. This workshop is designed to provide participants with an in-depth understanding of NGS technology, covering both theoretical aspects and hands-on experience with NGS workflows, including sample preparation, sequencing, and data analysis.

Workshop Highlights:

• Scientific Lectures: Expert-led sessions will introduce participants to the fundamentals of NGS, exploring its technological principles and applications in areas such as genomics, transcriptomics, epigenomics, and precision diagnostics. Participants will learn how NGS is transforming research and clinical workflows, with real-world case studies and insights into its utility in diagnosing genetic disorders, cancers, and infectious diseases.
• Hands-on Practical Sessions: Participants will gain direct experience with the complete NGS workflow, from sample preparation to data analysis. These sessions will cover:
  • Library Preparation: Detailed instructions on preparing DNA/RNA libraries, including quality checks, adapter ligation, and amplification steps.
  • Sequencing Workflow: Guidance on setting up sequencing runs, with an overview of commonly used platforms (e.g., Illumina, Ion Torrent).
  • Data Analysis: Training on bioinformatics pipelines, including alignment, variant calling, and annotation. Participants will learn how to interpret sequencing data and troubleshoot common challenges, ensuring high-quality and reliable results.
• Clinical and Research Applications: The workshop will focus on correlating NGS data with clinical or research outcomes, emphasizing the interpretation of results for diagnostic purposes and research innovations.

Detailed Agenda:

• Introduction to NGS: Principles, platforms, and applications in research and diagnostics
• Sample Preparation and Quality Control: Best practices for DNA/RNA extraction and quality assessment
• Library Preparation: Adaptor ligation, amplification, and indexing
• Sequencing Workflow: Setup, run monitoring, and basic troubleshooting
• Hands-on Practicals:
  • Sample and Library Preparation
  • Sequencing Run Setup and Overview
  • Raw Data Acquisition and Quality Check
• Bioinformatics Analysis:
  • Alignment and Mapping of Sequencing Reads
  • Variant Calling, Annotation, and Filtering
  • Data Visualization and Interpretation
• Clinical and Research Case Studies: Real-world examples of NGS applications, focusing on genetic testing, cancer diagnostics, and infectious disease genomics.

Key Learning Outcomes:

• Master the principles and workflow of NGS, from sample preparation to data interpretation.
• Gain proficiency in library preparation and sequencing setup, ensuring high-quality data.
• Develop skills in bioinformatics, including using tools for alignment, variant analysis, and data visualization.
• Understand how to apply NGS data in clinical and research contexts for impactful results.

Target Audience: This workshop is ideal for researchers, laboratory scientists, clinicians, and bioinformaticians working in genetics, oncology, infectious diseases, and other areas where NGS technology is transforming the landscape of precision medicine.