Introduction:

We are delighted to invite you to a comprehensive workshop on Fluorescence In Situ Hybridization (FISH) specifically tailored for applications in hematologic malignancies. This workshop is designed to equip participants with both theoretical knowledge and practical skills in FISH techniques, focusing on its critical role in diagnosing and characterizing hematologic disorders.

Workshop Highlights:

• Scientific Lectures: The workshop begins with in-depth lectures covering the fundamentals of FISH technology, including the principles behind fluorescence labelling and hybridization, and the various applications of FISH in hematologic malignancies. Participants will explore how FISH aids in identifying chromosomal abnormalities, gene rearrangements, and other genomic alterations relevant to blood cancers.
• Hands-on Practical Sessions: Participants will engage in hands-on sessions throughout the workshop, where they will learn the complete FISH workflow, from sample preparation to result interpretation. These sessions include:
  • Sample Preparation Techniques: Step-by-step guidance on preparing specimens for FISH analysis, with a focus on handling bone marrow and peripheral blood samples for accurate cytogenetic assessment.
  • Probe Selection and Hybridization: Training on selecting appropriate FISH probes for detecting specific genomic abnormalities. Participants will learn to set up hybridization reactions, ensuring optimal probe-target binding for clear signal detection.
  • Signal Detection and Analysis: Instruction on signal detection using fluorescence microscopy, with tips on troubleshooting and optimizing fluorescence signals for high-quality images.
• Data Interpretation and Clinical Correlation: The workshop concludes with a focus on interpreting FISH results and understanding how these findings correlate with clinical data. Participants will learn to analyze chromosomal abnormalities and other relevant markers to draw meaningful conclusions for patient diagnosis, prognosis, and treatment planning.

Detailed Agenda:

• Introduction to FISH: Principles, methodology, and clinical applications in hematologic malignancies
• Sample Preparation and Processing: Techniques for preparing blood and bone marrow samples for FISH
• Probe Selection and Hybridization: Guidance on selecting and applying FISH probes
• Signal Detection and Analysis: Hands-on microscopy sessions for signal observation and analysis
• Hands-on Practicals:
  • Sample Processing and Pretreatment
  • FISH Probe Hybridization
  • Post-Hybridization Washes for Signal Clarity
  • Signal Detection and Visualization under the Microscope

By the end of the workshop, participants will gain practical experience and confidence in performing FISH for hematologic malignancies. They will also have the opportunity to discuss specific case studies and explore ways to apply FISH techniques in their diagnostic or research settings.

Target Audience: This workshop is ideal for cytogeneticists, haematologists, laboratory technicians, and researchers involved in diagnostics and research on hematologic malignancies.