The workshop aims to provide participants with comprehensive, hands-on experience in the latest advancements of Droplet Digital PCR (ddPCR) technology. Designed for both beginners and experienced researchers, this workshop will cover the essential theoretical background and practical skills needed to harness ddPCR for highly sensitive and precise nucleic acid quantification.

Workshop Highlights:

• Scientific Lectures: Led by experts in the field, these sessions will cover the principles and applications of ddPCR, including an overview of its unique partitioning and detection techniques. Participants will gain insight into how ddPCR can be utilized for various applications such as rare mutation detection, copy number variation analysis, and absolute quantification of target nucleic acids.
• Practical Sessions: Participants will engage in hands-on practical sessions that encompass the complete ddPCR workflow. This includes:
  • Sample Preparation: Detailed instruction on preparing DNA/RNA samples for ddPCR, with a focus on optimizing input quality and quantity for accurate results.
  • Droplet Generation and PCR Setup: Step-by-step guidance on droplet generation and PCR reaction setup, highlighting best practices to ensure consistency and minimize errors.
  • Data Analysis: Training on the interpretation of ddPCR data, including the analysis of droplet counts, threshold setting, and quantification. Participants will also be introduced to software tools used for data analysis and learn troubleshooting tips for handling challenging samples or unexpected results.

By the end of the workshop, participants will have acquired the knowledge and skills necessary to implement ddPCR in their research confidently. They will also have the opportunity to discuss specific research applications and explore ways to integrate ddPCR into their existing workflows for more accurate and reliable molecular analysis.

Target Audience: This workshop is ideal for researchers, laboratory technicians, and clinicians involved in molecular diagnostics, genetic research, oncology, virology, and other fields where precise nucleic acid quantification is critical.